multis one step cloning kit (Vazyme Biotech Co)
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multis one step cloning kit
Multis One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1454 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multis+one+step+cloning+kit/ClonExpress+MultiS+One+Step+Cloning+Kit/pmc12995870-48-40-45
Average 97 stars, based on 1454 article reviews
Multis One Step Cloning Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 1454 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multis+one+step+cloning+kit/ClonExpress+MultiS+One+Step+Cloning+Kit/pmc12995870-48-40-45
Average 97 stars, based on 1454 article reviews
multis one step cloning kit - by Bioz Stars,
2026-09
97/100 stars
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Construct:Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: The target genes were amplified using PrimeSTAR Max DNA Polymerase (Takara #R045) with specific primers synthesized by Azenta Life Sciences ( ). .. To construct the initial linker recombinant plasmid, the N-terminal protein and the C-terminal sfGFP were cloned into the pUC19 vector backbone linked by the GS linker (GGGGGSGGGGSGGGGSGGGGSLDPGGGGSG, we define it as the experimental wild-type linker) [ ] using a ClonExpress Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library Article Snippet: .. Plasmids were constructed via recombination using the ClonExpress Article Title: Transcriptional Regulation of Starch Biosynthesis in Sorghum Grain by a MIKC-Type MADS-Box Transcription Factor: An In Vitro Analysis. Article Snippet: .. All constructs were generated using the ClonExpress® Recombinant:Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: The target genes were amplified using PrimeSTAR Max DNA Polymerase (Takara #R045) with specific primers synthesized by Azenta Life Sciences ( ). .. To construct the initial linker recombinant plasmid, the N-terminal protein and the C-terminal sfGFP were cloned into the pUC19 vector backbone linked by the GS linker (GGGGGSGGGGSGGGGSGGGGSLDPGGGGSG, we define it as the experimental wild-type linker) [ ] using a ClonExpress Plasmid Preparation:Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: The target genes were amplified using PrimeSTAR Max DNA Polymerase (Takara #R045) with specific primers synthesized by Azenta Life Sciences ( ). .. To construct the initial linker recombinant plasmid, the N-terminal protein and the C-terminal sfGFP were cloned into the pUC19 vector backbone linked by the GS linker (GGGGGSGGGGSGGGGSGGGGSLDPGGGGSG, we define it as the experimental wild-type linker) [ ] using a ClonExpress Article Title: Global transcription factors analyses reveal hierarchy and synergism of regulatory networks and master virulence regulators in Pseudomonas aeruginosa Article Snippet: .. Briefly, for the VSVG-tagged, the ORF was amplified by PCR from the PAO1 genome and cloned into pAK1900 plasmid by HindIII/BamHI for the overexpressed TFs through HindIII site by using ClonExpress Clone Assay:Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: The target genes were amplified using PrimeSTAR Max DNA Polymerase (Takara #R045) with specific primers synthesized by Azenta Life Sciences ( ). .. To construct the initial linker recombinant plasmid, the N-terminal protein and the C-terminal sfGFP were cloned into the pUC19 vector backbone linked by the GS linker (GGGGGSGGGGSGGGGSGGGGSLDPGGGGSG, we define it as the experimental wild-type linker) [ ] using a ClonExpress Article Title: Global transcription factors analyses reveal hierarchy and synergism of regulatory networks and master virulence regulators in Pseudomonas aeruginosa Article Snippet: .. Briefly, for the VSVG-tagged, the ORF was amplified by PCR from the PAO1 genome and cloned into pAK1900 plasmid by HindIII/BamHI for the overexpressed TFs through HindIII site by using ClonExpress Cloning:Article Title: Functional customization of peptide linkers in fusion proteins through multimodal deep learning approach Article Snippet: The target genes were amplified using PrimeSTAR Max DNA Polymerase (Takara #R045) with specific primers synthesized by Azenta Life Sciences ( ). .. To construct the initial linker recombinant plasmid, the N-terminal protein and the C-terminal sfGFP were cloned into the pUC19 vector backbone linked by the GS linker (GGGGGSGGGGSGGGGSGGGGSLDPGGGGSG, we define it as the experimental wild-type linker) [ ] using a ClonExpress Article Title: From Respiration to Secondary Metabolism: A Heme A Synthase-Mediated Regulatory Network Expands Indolizidine Chemical Space in Fungi Article Snippet: .. The ClonExpress Article Title: Formate-driven efficient pyruvate biosynthesis in Vibrio natriegens Article Snippet: PCR reactions were performed with KOD Plus Neo or KOD FX DNA polymerases (Toyobo Co., Japan). .. Multiple DNA fragments were assembled using the ClonExpress Article Title: A versatile CRISPR-associated transposases-mediated toolkit for transcriptional regulation library Article Snippet: .. Plasmids were constructed via recombination using the ClonExpress Article Title: Transcriptional Regulation of Starch Biosynthesis in Sorghum Grain by a MIKC-Type MADS-Box Transcription Factor: An In Vitro Analysis. Article Snippet: .. All constructs were generated using the ClonExpress® Article Title: Analysis of bidirectional promoter activity and structure of the large intergenic region (LIR) in mulberry crinkle leaf virus. Article Snippet: .. The two target fragments were reclaimed using a SanPrep Column DNA Gel Extraction Kit (Sangon Biotech, Shanghai, China) and were ligated into Sal I-linearized pCAMBIA2300 using the ClonExpress Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress Article Snippet: .. The mutant cgl1590 fragment was amplified from the genomic DNA of C. glutamicum ATCC 13032 using the primer pairs cgl1590 750insG -F1/ cgl1590 750insG - R1, cgl1590 750insG -F2/ cgl1590 750insG -R2, and then ligated with the Bam HI-linearized pK18 mobsacB using the ClonExpress Article Title: Global transcription factors analyses reveal hierarchy and synergism of regulatory networks and master virulence regulators in Pseudomonas aeruginosa Article Snippet: .. Briefly, for the VSVG-tagged, the ORF was amplified by PCR from the PAO1 genome and cloned into pAK1900 plasmid by HindIII/BamHI for the overexpressed TFs through HindIII site by using ClonExpress Generated:Article Title: Transcriptional Regulation of Starch Biosynthesis in Sorghum Grain by a MIKC-Type MADS-Box Transcription Factor: An In Vitro Analysis. Article Snippet: .. All constructs were generated using the ClonExpress® Gel Extraction:Article Title: Analysis of bidirectional promoter activity and structure of the large intergenic region (LIR) in mulberry crinkle leaf virus. Article Snippet: .. The two target fragments were reclaimed using a SanPrep Column DNA Gel Extraction Kit (Sangon Biotech, Shanghai, China) and were ligated into Sal I-linearized pCAMBIA2300 using the ClonExpress Mutagenesis:Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress Article Snippet: .. The mutant cgl1590 fragment was amplified from the genomic DNA of C. glutamicum ATCC 13032 using the primer pairs cgl1590 750insG -F1/ cgl1590 750insG - R1, cgl1590 750insG -F2/ cgl1590 750insG -R2, and then ligated with the Bam HI-linearized pK18 mobsacB using the ClonExpress Amplification:Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress Article Snippet: .. The mutant cgl1590 fragment was amplified from the genomic DNA of C. glutamicum ATCC 13032 using the primer pairs cgl1590 750insG -F1/ cgl1590 750insG - R1, cgl1590 750insG -F2/ cgl1590 750insG -R2, and then ligated with the Bam HI-linearized pK18 mobsacB using the ClonExpress Article Title: Global transcription factors analyses reveal hierarchy and synergism of regulatory networks and master virulence regulators in Pseudomonas aeruginosa Article Snippet: .. Briefly, for the VSVG-tagged, the ORF was amplified by PCR from the PAO1 genome and cloned into pAK1900 plasmid by HindIII/BamHI for the overexpressed TFs through HindIII site by using ClonExpress Polymerase Chain Reaction:Article Title: Global transcription factors analyses reveal hierarchy and synergism of regulatory networks and master virulence regulators in Pseudomonas aeruginosa Article Snippet: .. Briefly, for the VSVG-tagged, the ORF was amplified by PCR from the PAO1 genome and cloned into pAK1900 plasmid by HindIII/BamHI for the overexpressed TFs through HindIII site by using ClonExpress |